Association of GSTM1 and GSTT1 polymorphisms with DNA damage in coal-tar workers

作者：Giri Shiv Kumar; Yadav Anita^*; Kumar Anil; Dev Kapil; Gupta Ranjan; Aggarwal Neeraj; Seth Neena; Gautam Sanjeev Kumar

来源：Science of the Total Environment, 2011, 409(20): 4465-4469.

DOI：10.1016/j.scitotenv.2011.07.009

• Polycyclic aromatic hydrocarbon
• GSTM1
• GSTT1
• Alkaline comet assay
• DNA damage
• Tail moment

DNA damage was evaluated by alkaline comet assay in peripheral blood lymphocytes of 115 coal-tar workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs) and 105 control subjects. The effect of polymorphisms of glutathione S-transferase (GST) genotypes on the DNA damage was assessed. The mean tail moment (TM) value in the coal-tar workers was significantly higher as compared to the control subjects (12.06 +/- 0.55 versus 0.44 +/- 0.31; P<0.05). No significant association (P>0.05) between the GSTT1 and GSTM1 genotypes and the TM values was found, however highest mean rank TM value was reported in GSTM1 null and GSTT1 null genotypes in both control and exposed subjects. Our results suggest that there is increased DNA damage in coal-tar workers due to PAHs exposure. Polymorphisms in GSTM1 and GSTT1 genes do not show significant effect (P>0.05) on DNA damage.

• 出版日期2011-9-15