Application of BOLD MRI and DTI for the Evaluation of Renal Effect Related to Viscosity of Iodinated Contrast Agent in a Rat Model

作者：Wang, Yi; Ren, Ke^*; Liu, Yi; Sun, Wen-ge; Wang, Jia-huan; Zhang, Xin; Wu, Cheng-hua

来源：Journal of Magnetic Resonance Imaging, 2017, 46(5): 1320-1331.

DOI：10.1002/jmri.25683

• MAGNETIC-RESONANCE ELASTOGRAPHY
• REVISED ATLANTA CLASSIFICATION
• CHRONIC HEPATITIS-B
• ACUTE-PANCREATITIS
• LIVER STIFFNESS
• CT
• DIAGNOSIS
• FIBROSIS
• DISEASE

Purpose: To evaluate the effects of viscosity of contrast agent (CA) on intrarenal oxygenation and diffusion as measured by blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI) and diffusion tensor imaging (DTI) in a rat model. @@@ Materials and Methods: Radiocontrast iodixanol formulated in three viscosities were designated 270, 320, and 350 (mg iodine/mL). Sixty-three male Wistar rats were divided into four groups. Saline and iodixanol (4 g iodine/kg) were administered. MR images were acquired on a 3.0T scanner at baseline and at 1 hour, 24 hours, 48 hours, and 72 hours postinjection of solutions. BOLD-MRI was performed with a multiple gradient-recalled-echo sequence. The changes in R2*, apparent diffusion coefficient (ADC), fractional anisotropy (FA), histology, and hypoxia-inducible factor-1 alpha (HIF-1 alpha) immunoexpression were evaluated. The R2*, ADC, and Delta FA values were normalized to baseline to calculate Delta R2*, Delta ADC, and Delta FA. @@@ Results: Compared with baseline levels, distinct elevation of Delta R2* (P < 0.05) and obvious decrease in Delta ADC (P < 0.01) and DFA (P < 0.05) were observed in all the anatomical compartments at 1 hour after administration of CA. The absolute values in Delta R2*, Delta ADC, and Delta FA increased with increases in CA viscosity, and differed significantly between the CA groups in renal cortex (CO), outer stripe of outer medulla (OSOM), and inner stripe of outer medulla (ISOM) (all P < 0.05). A significant positive correlation was observed between Delta R2* and HIF-1 alpha expression (P < 0.001, r50.75). Significant negative correlations were observed between Delta ADC, Delta FA, and pathologies in CO, OSOM, ISOM (all P < 0.001, r=-0.68-0.87; all P < 0.001, r=-0.60-0.66). @@@ Conclusion: The effect of CA viscosity on intrarenal oxygenation and diffusion was viscosity-dependent, and was identified using BOLD-MRI and DTI.

• 出版日期2017-11
• 单位中国医科大学