Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920

作者：Lai Zee Wei; Rahim Raha Abdul; Ariff Arbakariya; Mohamad Rosfarizan^*

来源：African Journal of Microbiology Research, 2011, 5(15): 2114-2123.

• Streptococcus spp.
• hyaluronic acid
• helical ribbon impeller
• carbon/nitrogen

The effects of medium formulation and impeller design (Rushton turbine and helical ribbon) on hyaluronic acid (HA) biosynthesis by Streptococcus zooepidemicus ATCC 39920 were investigated using a 2 L stirred-tank bioreactor. The effect of different glucose concentrations (20, 30, 50 and 60 g/L), nitrogen sources ((NH(4))(2)S(2)O(8), (NH(4))(2)PO(4), yeast extract, and tryptone) and carbon/nitrogen ratios on the growth of the strain and on HA biosynthesis were initially investigated. Organic nitrogen sources (yeast extract and tryptone) were proven to be favourable in media for HA biosynthesis compared to inorganic nitrogen sources. About 2.442 g/L of HA with a high molecular weight (4.36 x 10(6) Da) was synthesised at an optimal C/N of 2.5: 1 (using a mixture of yeast extract and tryptone) in a 2 L stirred-tank bioreactor equipped with a Rushton turbine impeller. When using an optimal medium formulation at equal HA production levels, the helical ribbon impeller resulted in a higher molecular weight of HA (5.20 x 10(6) Da) compared to the Rushton turbine impeller.

• 出版日期2011-8-4
• 单位中国林业科学研究院热带林业研究所