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摘要
The digestion of polysaccharides from the wheat cultivars Caphorn and Isengrain was investigated, and the efficiency of an enzyme preparation was tested using the TNO gastrointestinal model (TIM-1). The apparent digestibility (AD) of carbohydrates was determined based on the measurement of organic matter (OM), total monosaccharides, reducing ends (RE) and end products (EP: glucose, maltose and xylobiose). The AD of the OM from Caphorn and Isengrain measured using caecectomised cockerels did not differ from that measured using TIM-1: 72.0 (SD 2.6) v. 70.6 (SD 0.6)% for Caphorn (P=0.580) and 73.0 (SD 2.3) v. 71.1 (SD 1.9)% for Isengrain (P=0.252). After the 6 h TIM-1 digestion, 41.4-58.9% of the OM, RE and EP were recovered from the jejunal compartment and 18.3-27.1% from the ileal compartment, while ileal deliveries and digestive residues constituted the remainder. A commercial enzyme cocktail tested at 0.2 mu l/g of wheat improved TIM-1 digestibility of Caphorn and Isengrain polysaccharides: 3.9% (P=0.0203) and 3.4% (P=0.0058) based on the OM; 9.7% (P<0.0001) and 3.1% (P=0.031) based on the total glucose; 47.2% (P<0.0001) and 14.2% (P=0.0004) based on the RE, respectively. The enzyme cocktail improved the release of the EP for Caphorn (3.8%, P=0.008) but not for Isengrain (-0.8%, P=0.561). The higher efficiency of the enzyme supplementation on the digestion of Caphorn polysaccharides compared with Isengrain seems to be linked to the higher soluble carbohydrate contents and/or less ramified arabinoxylan of Caphorn.
- 出版日期2011-7
