Ability of Clostridium butyricum to inhibit Escherichia coli-induced apoptosis in chicken embryo intestinal cells

作者：Gao, Quanxin; Qi, Lili; Wu, Tianxing; Wang, Jinbo^*

来源：Veterinary Microbiology, 2012, 160(3-4): 395-402.

DOI：10.1016/j.vetmic.2012.06.009

• Clostridium butyricum
• Escherichia coli
• Chicken embryo intestinal cells
• Apoptosis
• Adhesion

The beneficial effects of Clostridium butyricum in the treatment of intestinal inflammatory disorders are well known. However, it is not fully understood how such bacteria inhibit pathogen-induced intestinal diseases. For this purpose, we investigated the effects of C butyricum and its spent culture supernatants (SCS) on Escherichia coli (EHEC) growth and adherence to chicken embryo intestinal cells (CEICs). We also evaluated the potential of C butyricum to inhibit EHEC-induced apoptosis in CEICs. C butyricum and its SCS exhibited significant inhibitory activity on EHEC growth and adherence to CEICs. C butyricum also showed a significant inhibitory effect on EHEC-induced apoptosis by modulating the expression of XIAP (X-linked inhibitor of apoptosis protein), BclXL (B-cell lymphoma-extra large), FAS, Bcl2 (B-cell leukemia/lymphoma-2), BAX (Bcl-2-associated X protein), P53 (Tumor protein 53) and via inhibition of caspase-9 and caspase-3 activation. These results together indicate that C butyricum possesses the ability to prevent EHEC-induced intestinal disorders both directly, through inhibiting EHEC viability, and indirectly, via medicating EHEC-induced apoptosis. These observations may help explain the beneficial properties of C butyricum. Furthermore, our data is novel in the case of poultry and the manner in which C butyricum prevents the EHEC-induced apoptosis provides supportive information for the treatment of colibacillosis in poultry.

• 出版日期2012-12-7
• 单位浙江大学; 浙江大学宁波理工学院