科研之友
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摘要
Dendritic cells (DCs) as professional antigen presenting cells, are important in the initiation of the primary immune response. The present study compared the morphology, phenotypes and function between monocyte-derived human DCs produced from a conventional culturing system containing granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (IL-4-DC) and DCs generated by the stimulation of GM-CSF and interferon (IFN)-alpha (IFN-DC). When compared with IL-4-DC in morphology, IFN-DC contained more organelles, including endoplasmic reticulum and myelin figures, whereas mature (m)IL-4-DC contained more vacuoles in the cells. The spikes of IFN-DC were shorter and thicker. The expression of phenotypes between immature IFN-DC and IL-4-DC were diverse. Following maturation with tumor necrosis factor-alpha, IFN-DC and IL-4-DC upregulated the expression of cluster of differentiation (CD) 11c and CD83. Conversely, immature IFN-DC and IL-4-DC secreted few inflammatory cytokines including interleukin (IL)-18, IL-23, IL-12p70, IL-1 beta and anti-inflammatory IL-10. Following maturation, large amounts of the cytokines were secreted by these two DCs and mIFN-DC secreted more cytokines compared with mIL-4-DC in general. Furthermore, immature IFN-DC and IL-4-DC loaded with cytomegalovirus (CMV)-pp65 protein were unable to induce the priming of T cells, as evaluated by the intracellular staining with IFN-gamma. Notably, mature DCs exhibited the ability to present CMV-pp65 protein and activate T cells. The mIFN-DC activated a greater proportion of autologous CD4(+) T cells (0.91 vs. 0.31%, P<0.001) and CD8(+) T cells (0.90 vs. 0.48%, P<0.001) to secret IFN-gamma compared with mIL-4-DC. The results suggested that the morphology, phenotypes and cytokine secretion of IFN-DC and IL-4-DC were diverse. The mIFN-DC were more effective in priming and cross-priming T cells when compared with IL-4-DC.
