Anti-Inflammatory Activity of Triterpenes Isolated from Protium paniculatum Oil-Resins

作者：de Almeida Patricia D O; Boleti Ana Paula de A; Ruediger Andre Luis; Lourenco Geane A; da Veiga Junior Valdir Florencio; Lima Emerson S^*

来源：Evidence-Based Complementary and Alternative Medicine, 2015, 2015: 293768.

DOI：10.1155/2015/293768

Protium is the main genus of the Burseraceae family and one of the most common genera in South America, with an important species called "breu." Gum and oil-resins of this species are used as tonic and stimulant and for the treatment of ulcers and inflammation. The present study aims to isolate and investigate the anti-inflammatory activity of triterpene compounds isolated from oil-resin of Protium paniculatum. The pentacyclic triterpenes alpha,beta-amyrin, acetylated alpha,beta-amyrin alpha,beta-amyrone, and brein/maniladiol did not alter the viability of murine J774 macrophages (IC50 > 20 mu g/mL), with the exception of mixture of brein/maniladiol which showed moderate cytotoxic activity. Also it was observed that compounds at 10 mu g/mL inhibitedmore than 80% of production of NO center dot, although only alpha,beta-amyrin was able to inhibit the production of TNF-alpha (52.03 +/- 2.4%). The compounds inhibited the production of IL-6 and induced the production of IL-10 inmurine J774 macrophages stimulated by LPS alpha,beta-Amyrone inhibited the expression of COX-2 and also inhibited the formation of paw or ear edema in rats and mice, having a quick and immediate effect. This study may provide the basis for future investigations on the therapeutic role of alpha,beta-amyrone in treating inflammation.

• 出版日期2015