Nanosized tin dioxide - Unexplored carrier for lipase immobilization

作者：Guncheva Maya^*; Dimitrov Momtchil; Zhiryakova Diana

来源：Catalysis Communications, 2011, 16(1): 205-209.

DOI：10.1016/j.catcom.2011.10.007

• Lipase
• Immobilization
• Nanosized tin dioxide
• SBA-15
• Isoamyl acetate

A lipase from Rhizopus delemar (RhDL) was adsorbed on two tin dioxides with different textural characteristics (nanoSnO(2)-A-RhD and nanoSnO(2)-B-RhD). All biochemical characteristics obtained for them were compared with those for RhDL on silica. NanoSnO(2)-A exhibited the highest protein loading capacity (75%), however, the highest specific activity was measured for nanoSnO(2)-B-RhD (33.8 U/mg prot.). The immobilization enhanced thermal-, pH- and solvent stability of RhDL For example, nanoSnO(2)-A-RhD is still fully active after one-hour heating at 60 degrees C and preserved 70% of its activity at pH 9. When applied in the isoamyl acetate synthesis, a complete conversion of substrates was obtained with nanoSnO(2)-A-RhD in hexane and SBA-15-RhD in hexadecane for 12 h. The performance of the three biocatalysts in several consecutive cycles was estimated as well.

• 出版日期2011-11-30