Mitochondria-specific imaging in living cells with two-photon absorption small molecule containing amino groups

作者：Guan, Rongting; Wang, Hui; Zhang, Qiong^*; Wu, Jieying^*; Tian, Yupeng

来源：Dyes and Pigments, 2017, 136: 473-479.

DOI：10.1016/j.dyepig.2016.09.012

• Amino
• Crystal structures
• Z-scan
• Two-photon microscopy
• Two-photon absorption cross section
• Zebra fish

Two-photon fluorescent probes have emerged as promising molecular tools for imaging subcellular organelles. Here, the facile synthesis of three novel nitro molecules (Z1-Z3) with lager two-photon absorption cross-section and three novel amino molecules (Ll-L3) based on mitochondrial probes with two-photon absorption are presented. Their photophysical properties have been investigated both experimentally and theoretically. The study of nonlinear optical property revealed that Z1-Z3 possess more excellent two-photon absorption (2PA) cross-section values than LI-L3 and the 2PA cross-section values were enhanced with increasing electron-donating strength of the end group when fixed the opposite group (nitro or amino). Furthermore, in consideration of the water solubility and fluorescent intensity, L1-L3 possessing high specificity for mitochondrial localization were applied to biology, which is advantageous in comparison with commercially available mitochondrial trackers. Due to their low cytotoxicity, these small molecules L1-13 offer a promising platform to directly monitor mitochondria in living cells and zebra fish.

• 出版日期2017-1
• 单位安徽大学