Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly

作者：Bulati Matteo; Buffa Silvio; Martorana Adriana; Candore Giuseppina; Lio Domenico; Caruso Calogero; Colonna Romano Giuseppina^*

来源：Experimental Gerontology, 2014, 54: 123-129.

DOI：10.1016/j.exger.2013.12.011

• B lymphocytes
• Chemokine receptors
• Inflamm-aging
• Elderly
• IL-21
• Granzyme B

The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG(+) IgD(-) CD27(-), double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of naive/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on naive/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. %26lt;br%26gt;Our data demonstrate that in the elderly, naive/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of %26quot;inflamm-aging%26quot;. In particular IgG(+)IgD(-)CD27(-) DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.

• 出版日期2014-6