Activation of myeloid dendritic cells by deoxynucleic acids from Cordyceps sinensis via a Toll-like receptor 9-dependent pathway

作者：Xiao, Gang; Miyazato, Akiko; Abe, Yuzuru; Zhang, Tiantuo; Nakamura, Kiwamu; Inden, Ken; Tanaka, Misuzu; Tanno, Daiki; Miyasaka, Tomomitsu; Ishii, Keiko; Takeda, Kiyoshi; Akira, Shizuo; Saijo, Shinobu; Iwakura, Yoichiro; Adachi, Yoshiyuki; Ohno, Naohito; Yamamoto, Natsuo; Kunishima, Hiroyuki; Hirakata, Yoichi; Kaku, Mitsuo; Kawakami, Kazuyoshi^*

来源：Cellular Immunology, 2010, 263(2): 241-250.

DOI：10.1016/j.cellimm.2010.04.006

• Cordyceps sinensis
• DNA
• Dendritic cells
• TLR9
• Cytokines
• CD40

The mechanism by which host cells recognize Cordyceps sinensis. a Chinese herbal medicine that is known to exhibit immunomodulating activity, remains poorly understood In this study, we investigated whether the DNA of this fungus could activate mouse bone marrow-derived dendritic cells (BM-DCs) Upon stimulation with C sinensis DNA, BM-DCs released IL-12p40 and TNF-alpha and expressed CD40 Cytokine production and CD40 expression were attenuated by chloroquin and bafilomycin A. Activation of BM-DCs by C sinensis DNA was almost completely abrogated in TLR9KO mice According to a luciferase reporter assay. C sinensis DNA activated NF-kappa B in HEK293T cells transfected with the TLR9 gene Finally, a confocal microscopic analysis showed that C. sinensis DNA was co-localized with CpG-ODN and partly with TLR9 and LAMP-1, a late endosomal marker, in BM-DCs Our results demonstrated that C sinensis DNA caused activation of BM-DCs in a TLR9-dependent manner.

• 出版日期2010
• 单位河北医科大学; 中山大学