UV-induced differential gene expression in rice cultivars analyzed by SSH

作者：Fang Chang Xun; Wu Xing Chun; Zhang Hai Li; Jun Xiong; Wu Wen Xiang; Lin Wen Xiong^*

来源：Plant Growth Regulation, 2009, 59(3): 245-253.

DOI：10.1007/s10725-009-9411-y

• UV-B resistance
• Oryza sativa
• Gene expression
• Suppression subtractive hybridization
• Real-time quantitative reverse transcriptase polymerase chain reaction

The differences in gene expression between two rice genotypes-the ultraviolet-B (UV-B)-resistant cultivar Lemont and the UV-B-sensitive cultivar Dular-in response to irradiation with UV-B were studied. Suppression subtractive hybridization and real-time quantitative reverse transcriptase polymerase chain reaction were used in the analysis. The results showed that enhanced UV-B radiation could induce differential expression of the genes related to defense response. The expression of genes encoding HECT domain-containing protein, thylakoid-bound ascorbate peroxidase, receptor-like protein kinase, Rad6 (ubiquitin carrier protein), phenylalanine ammonia-lyase, 4-coumarate: CoA ligase and chalcone synthase was up-regulated to different extents in the two rice cultivars: The cultivar Lemont exhibited a higher increase in the gene expression compared with the cultivar Dular. A large difference was found in the expression pattern of the universal stress protein (USP): showing down-regulation in Dular but up-regulation in Lemont. Hence exposure to UV-B radiation has a significant effect on gene expression in rice. In response to UV exposure, a molecular response is initially induced in different UV-B-resistant rice cultivars. This response may activate secondary metabolic pathways, in turn increase the synthesis of UV-protective substances (flavonoids) to prevent UV-B damage and induce the transcription of the relevant mRNAs for repairing the damaged DNA.

• 出版日期2009-12
• 单位福建农林大学