Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection

作者：Guedes de Sa Keyla Santos; Pires Neto Orlando de Souza; Santana Barbara Brasil; Monteiro Gomes Samara Tatielle; Graca Amoras Ednelza da Silva; da Silva Conde Simone Regina; Demachki Samia; Azevedo Vania Nakauth; Almeida Machado Luiz Fernando; Martins Feitosa Rosimar Neris; Guimaraes Ishak Marluisa de Oliveira; Ishak Ricardo; Rosario Vallinoto Antonio Carlos

来源：Revista da Sociedade Brasileira de Medicina Tropical, 2015, 48(2): 136-142.

DOI：10.1590/0037-8682-0008-2015

• TLR3
• rs5743305
• rs3775291
• HCV
• HBV

Introduction: The present study investigated the prevalence of two single-nucleotide polymorphisms (SNPs) in the Toll-like receptor 3 (TLR3) gene in patients infected with hepatitis B virus (HBV) and hepatitis C virus (HCV). Methods: Samples collected from HCV (n = 74) and HBV (n = 35) carriers were subjected to quantitative real-time PCR (qPCR) to detect the presence of the SNPs rs5743305 and rs3775291 in TLR3 and to measure the following biomarkers: alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), and prothrombin time (PT). A healthy control group was investigated and consisted of 299 HCV- and HBV-seronegative individuals. Results: No significant differences in allele, genotype and haplotype frequencies were observed between the investigated groups, and no association was observed between the polymorphisms and histopathological results. Nevertheless, genotypes TA/AA (rs5743305) and GG (rs3775291) appear to be associated with higher levels of ALT (p<0.01), AST (p<0.05) and PT (p<0.05). In addition, genotypes TT (rs5743305; p<0.05) and GG (rs3775291; p<0.05) were associated with higher GGT levels. Conclusions: This genetic analysis revealed the absence of an association between the polymorphisms investigated and susceptibility to HBV and HCV infection; however, these polymorphisms might be associated with a greater degree of biliary damage during the course of HCV infection.

• 出版日期2015-4