A high effective NADH-ferricyanide dehydrogenase coupled with laccase for NAD(+) regeneration

作者：Wang, Jizhong; Yang, Chengli; Chen, Xing; Bao, Bingxin; Zhang, Xuan; Li, Dali^*; Du, Xingfan; Shi, Ruofu; Yang, Junfang; Zhu, Ronghui

来源：Biotechnology Letters, 2016, 38(8): 1315-1320.

DOI：10.1007/s10529-016-2106-3

• Coenzyme regeneration
• Coupled enzymes
• Laccase
• NAD(+)
• NADH dehydrogenase
• Potassium ferricyanide

To find an efficient and cheap system for NAD(+) regeneration
A NADH-ferricyanide dehydrogenase was obtained from an isolate of Escherichia coli. Optimal activity of the NADH dehydrogenase was at 45 A degrees C and pH 7.5, with a K (m) value for NADH of 10 mu M. By combining the NADH dehydrogenase, potassium ferricyanide and laccase, a bi-enzyme system for NAD(+) regeneration was established. The system is attractive in that the O-2 consumed by laccase is from air and the sole byproduct of the reaction is water. During the reaction process, 10 mM NAD(+) was transformed from NADH in less than 2 h under the condition of 0.5 U NADH dehydrogenase, 0.5 U laccase, 0.1 mM potassium ferricyanide at pH 5.6, 30 A degrees C
The bi-enzyme system employed the NADH-ferricyanide dehydrogenase and laccase as catalysts, and potassium ferricyanide as redox mediator, is a promising alternative for NAD(+) regeneration.

• 出版日期2016-8
• 单位南京理工大学; 辽宁省农业科学院