Development of a PCR-based SNP marker system for effective selection of kernel length and kernel elongation in rice

作者：Ramkumar G; Sivaranjani A K P; Pandey Manish K; Sakthivel K; Rani N Shobha; Sudarshan I; Prasad G S V; Neeraja C N; Sundaram R M; Viraktamath B C; Madhav M S^*

来源：Molecular Breeding, 2010, 26(4): 735-740.

DOI：10.1007/s11032-010-9492-3

• Kernel length
• Kernel elongation
• Oryza sativa
• GS3
• Basmati

Kernel length in rice (Oryza sativa L.) is controlled by various quantitative trait loci of which GS3 is the most important, being responsible for 80-90% of the variation in kernel length. A mutation in the second exon of this gene has been reported to be associated with maximum variations in the kernel length. We have developed a simple PCR-based marker system named DRR-GL which targets the functional nucleotide polymorphism at GS3. This marker system has the advantages that it is easy to use, saves time and cost, and is amenable for large-scale marker-assisted selection for the trait of kernel length. Validation of this marker in a segregating population and 152 rice varieties, which includes 30 elite basmati varieties, reveals its effective co-segregation and association with the traits of kernel length as well as kernel elongation after cooking. We recommend utilization of this simple, low-cost marker system in breeding programs targeted at improvement of key rice grain quality traits, kernel length and kernel elongation.

• 出版日期2010-12