Alleviation of hepatic injury by chrysin in cisplatin administered rats: Probable role of oxidative and inflammatory markers

作者：Rehman Muneeb U; Ali Nemat; Rashid Summya; Jain Tyan; Nafees Sana; Tahir Mir; Khan Abdul Quaiyoom; Lateef Abdul; Khan Rehan; Hamiza Oday O; Kazim Syed; Qamar Wajhul; Sultana Sarwat^*

来源：Pharmacological Reports, 2014, 66(6): 1050-1059.

DOI：10.1016/j.pharep.2014.06.004

• Cisplatin
• Inflammation
• Oxidative stress
• Hepatotoxicity

Background: Cisplatin is an effective and extensively used chemotherapeutic agent to treat range of malignancies, but its therapeutic use is limited because of dose-dependent nephrotoxicity and hepatotoxicity. Several published reports advocate that supplementation with antioxidant can influence cisplatin induced hepatic damage.
Method: In the present study the Wistar rats were subjected to concurrent prophylactic oral treatment of chrysin (25 and 50 mg/kg b.wt.) against the hepatotoxicity induced by intraperitoneal administration of cisplatin (7.5 mg/kg b.wt.). Efficacy of chrysin against the hepatotoxicity was evaluated in terms of biochemical estimation of antioxidant enzyme activities, histopathological changes and expression levels of molecular markers of inflammation.
Results: Chrysin ameliorated cisplatin-induced lipid peroxidation, xanthine oxidase activity, glutathione depletion, decrease in antioxidant (catalase, glutathione reductase, superoxide dismutase, giutathione peroxidase and glucose-6 phosphate dehydrogenase) and phase-II detoxifying (glutathione-S-transferase and quinone reductase) enzyme activities. Chrysin also attenuated expression of COX-2, iNOS and levels of NF kappa B and TNF-alpha, and hepatic tissue damage which were induced by cisplatin. Histological findings further supported the protective effects of chrysin against cisplatin-induced hepatic damage.
Conclusion: The results of the present study demonstrate that oxidative stress and inflammation are closely associated with cisplatin-induced toxicity and chrysin shows the protective efficacy against cisplatin-induced hepatotoxicity possibly via attenuating the oxidative stress and inflammatory response.

• 出版日期2014-12