Activation of Wnt3α/β-catenin signal pathway attenuates apoptosis of the cerebral microvascular endothelial cells induced by oxygen-glucose deprivation

作者：Zhang, Jianshui; Zhang, Junfeng; Qi, Cunfang; Yang, Pengbo; Chen, Xinlin; Liu, Yong^*

来源：Biochemical and Biophysical Research Communications, 2017, 490(2): 71-77.

DOI：10.1016/j.bbrc.2017.03.130

• Brain microvascular endothelial cells (BMECs)
• Wnt3 alpha/beta-catenin signal
• Oxygen-glucose deprivation (OGD)
• Apoptosis

Brain microvascular endothelial cells (BMECs) play vital roles in cerebral ischemia, during which many signal pathways mediate BMECs apoptosis. In this study, we explored the potential role of Wnt3 alpha/beta-catenin signal in BMECs apoptosis induced by ischemia. Here, we found that oxygen-glucose deprivation (OGD) could induce apoptosis of BMECs with Wnt3a mRNA expression decrease. Meanwhile, activation Wnt3 alpha/beta-catenin signal with exogenous Wnt3 alpha protein (100 ng/ml) or Lithium Chloride (LiCl, 4 mM) decreased significantly apoptosis of BMECs induced by OGD with increasing expression of Bcl-2 in the whole cell and beta-catenin in the nucleus. But, inhibition Wnt3 alpha/beta-catenin signal with DKK1 (100 ng/ml) or 2.4-diamino quinazoline (DQ, 0.2 mu M) increased apoptosis of BMECs with decreasing expression of Bcl-2. These results suggest that activation Wnt3 alpha/beta-catenin signal attenuate apoptosis of BMECs induced by ischemia.

• 出版日期2017-8-19
• 单位西安医学院; 西安交通大学; 青海大学