Molecular profiling of single circulating tumor cells from lung cancer patients

作者：Park Seung min; Wong Dawson J; Ooi Chin Chun; Kurtz David M; Vermesh Ophir; Aalipour Amin; Suh Susie; Pian Kelsey L; Chabon Jacob J; Lee Sang Hun; Jamali Mehran; Say Carmen; Carter Justin N; Lee Luke P; Kuschner Ware G; Schwartz Erich J; Shrager Joseph B; Neal Joel W; Wakelee Heather A; Diehn Maximilian; Nair Viswam S^*; Wang Shan X^*; Gambhir Sanjiv S^*

来源：Proceedings of the National Academy of Sciences, 2016, 113(52): E8379-E8386.

DOI：10.1073/pnas.1608461113

• circulating tumor cells
• microfluidics
• rare-cell sorting
• reverse transcription-PCR
• single-cell analysis

Circulating tumor cells (CTCs) are established cancer biomarkers for the "liquid biopsy" of tumors. Molecular analysis of single CTCs, which recapitulate primary and metastatic tumor biology, remains challenging because current platforms have limited throughput, are expensive, and are not easily translatable to the clinic. Here, we report a massively parallel, multigene-profiling nanoplatform to compartmentalize and analyze hundreds of single CTCs. After high-efficiency magnetic collection of CTC from blood, a single-cell nanowell array performs CTC mutation profiling using modular gene panels. Using this approach, we demonstrated multigene expression profiling of individual CTCs from non-small-cell lung cancer (NSCLC) patients with remarkable sensitivity. Thus, we report a high-throughput, multiplexed strategy for single-cell mutation profiling of individual lung cancer CTCs toward minimally invasive cancer therapy prediction and disease monitoring.

• 出版日期2016-12-27