Development of a Stable Isotope Dilution Assay for Tenuazonic Acid

作者：Asam Stefan; Liu Yang; Konitzer Katharina; Rychlik Michael^*

来源：Journal of Agricultural and Food Chemistry, 2011, 59(7): 2980-2987.

DOI：10.1021/jf104270e

• Alternaria
• mycotoxins
• tenuazonic acid
• stable isotope dilution assay
• SIDA
• LC-MS/MS

A stable isotope dilution assay (SIDA) for the Alternaria mycotoxin tenuazonic acid was developed. Therefore, [(13)C(6),(15)N]-tenuazonic acid was synthesized from [(13)C(6),(15)N]-isoleucine by Dieckmann intramolecular cyclization after acetoacetylation with diketene. The synthesized [(13)C(6),(15)N]-tenuazonic acid was used as the internal standard for determination of tenuazonic acid in tomato products by liquid chromatography tandem mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine. Method validation revealed a limit of detection of 0.1 mu g/kg and a limit of quantitation of 0.3 mu g/kg. Recovery was close to 100% in the range of 3-300 mu g/kg. Determination of tenuazonic acid in two samples of different tomato ketchups (naturally contaminated) was achieved with a coefficient of variation of 2.3% and 4.7%. Different tomato products (n = 16) were analyzed for their content of tenuazonic acid using the developed SIDA. Values were between 15 and 195 mu g/kg (tomato ketchup, n = 9), 363 and 909 mu g/kg (tomato paste, n = 2), and 8 and 247 mu g/kg (pureed tomatoes and comparable products, n = 5).

• 出版日期2011-4-13