Unilateral renal denervation and the renal kallikrein-bradykinin system in the rat

作者：Bohlender J^*; Nus**erger J; Amstutz C; Birkhauser F; Thalmann G N; Imboden H

来源：Annales de Cardiologie et d'Angeiologie, 2013, 62(3): 144-148.

DOI：10.1016/j.ancard.2013.04.014

• Bradykinin
• Kallikrein
• Measurement
• Plasma
• Tissue
• Kidney
• Denervation
• Renal nerve ablation
• Experimental hypertension

Aim. - The antihypertensive effect of renal denervation in hypertensive patients is partially explained by increased tubular natriuresis. To study the possible contribution of the kallikrein-kinin system (KKS) to this natriuretic effect in rats, we measured kallikrein activity (KA) and bradykinin concentrations (BK) in plasma and tissues. %26lt;br%26gt;Methods. - To measure KA, we adapted and validated an enzymatic assay that cleaves para-nitroaniline (pNA) from the tripeptide H-D-Pro-Phe-Arg-pNA. The coefficients of variation (CV) within- and between-assays were less than 8% for plasma and tissue KA (plasma n=6 and 13; tissue n=4). Linear results for serially diluted samples confirmed the assay specificity. Tissue BK determinations were based on an established assay for plasma BK: tissue was homogenized and kinins extracted in ethanol, and BK was isolated by high-performance (HPLC) liquid chromatography and quantitated by radioimmunassay. Within- and between-assay CV for plasma BK were 18% (n = 8 and n=35, respectively) and for BK in various tissues less than 16% (n=5-8). %26lt;br%26gt;Results. - In male Wistar rats (n=3), plasma BK was 8.2 +/- 6.6 fmol/naL (mean +/- SD), and tissue BK (fmol/g) in 14 tested organs varied between brain (14 +/- 3) and submaxillary gland (521 +/- 315). Six days after left-sided unilateral renal denervation, left renal tissue BK (89 +/- 9) was not different from right renal BK (75 +/- 23). Similarly, KA was comparable in the two kidneys (left 18.0 +/- 1.5, right 15.8 +/- 1.4 mu kat/g). %26lt;br%26gt;Conclusion. - Any possible effect of unilateral renal denervation on the kidney%26apos;s KKS would have to be bilateral.

• 出版日期2013-6