Alteration in Lipid and Protein Profiles of Ovarian Cancer Similarity to Breast Cancer

作者：Kang Sokbom; Lee Aera; Park Young Seung; Lee Seok Cheol; Park Sang Yoon; Han Sang Yun; Kim Kwang Pyo; Kim Young Hwan^*; Yoo Chong Woo; Kim Hark Kyun

来源：International Journal of Gynecological Cancer, 2011, 21(9): 1566-1572.

DOI：10.1097/IGC.0b013e318226c5f5

• Histology-directed MALDI
• Ovarian cancer
• Protein
• Lipid

This study was undertaken to evaluate protein and lipid profiles of ovarian cancer tissue samples. Twenty-three frozen ovarian cancer samples and 6 adjacent normal samples were analyzed using histology-directed, matrix-assisted laser desorption/ionization mass spectrometry. Sinapinic acid and 2, 5-dihydroxybenzoic acid/alpha-cyano-4-hydroxycinnamic acid were manually deposited on areas of each tissue section enriched in epithelial cells to identify protein and lipid profiles respectively, and mass spectra were acquired using a matrix-assisted laser desorption/ionization-time of flight instrument. Protein and lipid profiles classify 11 cancer and 3 adjacent normal samples in 100 random test sets with 92.9% median accuracy. Phosphatidylcholines {32: 3} [M + Na](+) (m/z = 750.66), {34: 1} [M + K](+) (m/z = 798.60), and {36: 2} [M + K](+) (m/z = 824.56) were found to be increased in ovarian cancer. Interestingly, breast cancer-associated changes in lipid and protein profiles were also found in ovarian cancer. Thus, protein and lipid profiles accurately distinguish ovarian cancer from adjacent normal tissue samples. Common cancer-associated alterations in lipid and protein profiles were identified between ovarian and breast cancers.

• 出版日期2011-12