A Highly Efficient ADH-Coupled NADH-Recycling System for the Asymmetric Bioreduction of Carbon-Carbon Double Bonds Using Enoate Reductases

作者：Tauber Katharina; Hall Melanie; Kroutil Wolfgang; Fabian Walter M F; Faber Kurt; Glueck Silvia M^*

来源：Biotechnology and Bioengineering, 2011, 108(6): 1462-1467.

DOI：10.1002/bit.23078

• cofactor recycling
• coupled-enzyme approach
• alcohol dehydrogenase
• asymmetric bioreduction of alkene
• enoate reductase

The asymmetric bioreduction of activated alkenes catalyzed by flavin-dependent enoate reductases from the OYE-family represents a powerful method for the production of optically active compounds. For its preparative-scale application, efficient and economic NADH-recycling is crucial. A novel enzyme-coupled NADH-recycling system is proposed based on the concurrent oxidation of a sacrificial sec-alcohol catalyzed by an alcohol dehydrogenase (ADH-A). Due to the highly favorable position of the equilibrium of ene-reduction versus alcohol-oxidation, the cosubstrate is only required in slight excess. Biotechnol. Bioeng. 2011;108: 1462-1467.

• 出版日期2011-6