Muscle specific nucleus ambiguus neurons isolation and culturing

作者：Hernandez Morato Ignacio^*; Pitman Michael J; Sharma Sansar

来源：Journal of Neuroscience Methods, 2016, 273: 33-39.

DOI：10.1016/j.jneumeth.2016.07.014

• Reinnervation
• Motoneurons
• Neuron culture
• Neuron isolation
• Recurrent laryngeal nerve

Background: Peripheral nerve injury leads to a regenerative state. However, the reinnervation process is highly non-selective. Growing axons are often misrouted and establish aberrant synapsis to abductor or adductor muscles. Determining the complex properties of abductor and adductor motoneurons in a neuron culture, may lay the groundwork for future studies on axon guidance, leading to a clinical treatment for a selective reinnervation.
New method: In the present study we develop a neuron culture protocol to isolate recurrent laryngeal nerve abductor and adductor motoneurons in order to study their unique properties.
Comparison with existing methods the best period to perform the present protocol for postnatal rat cranial motoneurons isolation was determined. In addition, the method allows identification of specific motoneurons from other primary motoneurons and interneurons within brainstem.
Conclusion: The present protocol will allow investigators to perform targeted and novel studies of the mechanisms of peripheral nerve regeneration.

• 出版日期2016-11-1