A magnetic nanoparticles-zinc oxide/zinc hexacyanoferrate hybrid film for amperometric determination of tyrosine

作者：Narang Jagriti; Chauhan Nidhi; Pundir Shikha; Pundir C S^*

来源：Bioprocess and Biosystems Engineering, 2013, 36(11): 1545-1554.

DOI：10.1007/s00449-013-0916-4

• Tyrosine
• Magnetic nanoparticles
• Zinc oxide/zinc hexacyanoferrate film
• Pt electrode
• Serum
• Phenylketonuria

A method is described for the construction of a highly sensitive amperometric sensor for the detection of tyrosine, employing a magnetic nanoparticles-zinc oxide/zinc hexacyanoferrate (Fe3O4NP-ZnO/ZnHCF) hybrid film electrodeposited on the surface of a Pt electrode as working electrode. The sensor is based on electrocatalytic mechanism initiated by electrochemical oxidation of the reduced form of the hybrid film at +0.2 V vs. Ag/AgCl followed by completion of chemical oxidation of tyrosine. The sensor showed optimum response within 2 s at pH 2. The working/linear range of the sensor was 0.02-2.76 mM with a detection limit of 4 mu M. The sensor measured tyrosine level in serum, a potential biomarker of phenylketonuria. The working electrode lost only 5 % of its initial activity, when stored at 4 A degrees C, after its regular use over a period of 100 days.

• 出版日期2013-11