Molecular cloning, recombinant expression, and antifungal functional characterization of the lipid transfer protein from Panax ginseng

作者：Cai, Kexin; Wang, Jiawen; Wang, Min; Zhang, Hui; Wang, Siming; Zhao, Yu^*

来源：Biotechnology Letters, 2016, 38(7): 1229-1235.

DOI：10.1007/s10529-016-2100-9

• Antifungal activity
• Lipid transfer proteins
• Panax ginseng
• Protein expression and purification

To establish an efficient expression system for a fusion protein GST-pgLTP (Lipid Transfer Protein) and to test its antifungal activity. @@@ The nucleotide sequence of LTP gene was obtained from Panax ginseng using RT-PCR. The ORF of the cDNA is 363 bp, codING for a protein OF 120 amino acids with a calculated MW of 12.09 kDa. The pgLTP gene with a His(6)-tag at the C-terminus was cloned into the pGEX-6p1 vector to generate a GST-fusion pgLTP protein construct that was expressed in Escherichia coli Rosetta. Following purification by Ni-NTA, the fusion protein exhibited antifungal activity against five fungi found in ginseng. @@@ The fusion protein GST-pgLTP has activity against a broad spectrum of phytopathogenic fungi, and can potentially be adapted for production to combat fungal diseases that affect P. ginseng.

• 出版日期2016-7
• 单位长春中医药大学