科研之友
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摘要
A genetic linkage map of cauliflower (Brassica oleracea var. botrytis) has been constructed based on AFLP and nucleotide binding site (NBS) markers, in order to identify potential molecular markers linked to important agronomic traits that could be useful for developing and improving the species. NBS profiling was first used to map resistance gene analogues (kGAs) in cauliflower (Brassica oleracea var. botrytis), which simultaneously allowed the amplification and mapping of genetic markers anchored in the conserved NBS encoding domain of plant disease resistance genes. At the same time, the AFLP method was also performed in this paper to construct an intervarietal genetic map of cauliflower. A total of 234 AFLP markers and 21 NBS markers were mapped in an F-2 Population derived by self-pollinating a single F-1 plant (a hybrid between "AD White Flower" and "C-8") based on seventeen AFLP primer combinations and two degenerate primer/enzyme combinations. The markers were mapped into nine major linkage groups, spanning 668.4 cM, with an average distance of 2.9 cM between adjacent mapped markers. Each of the linkage groups contained from 12 to 47 loci, and the distance between two consecutive loci ranged from 0 to 14.9 cM. The AFLP markers were well distributed throughout the nine linkage groups, and eight linkage groups for the NBS markers. Most NBS markers mapped in this study were organized in clusters, indicating that most of them could be real RGAs. The maps we have generated provide a firm basis for mapping agriculturally relevant traits, which will then open the way for application of a marker-assisted selection breeding strategy in this species.
- 出版日期2008-4
- 单位南开大学
