Nanometer-grooved topography stimulates trabecular bone regeneration around a concave implant in a rat femoral medulla model

作者：Klymov Alexey; te Riet Joost; Mulder Peter; Gardeniers Johannes G E; Jansen John A; Walboomers X Frank^*

来源：Nanomedicine: Nanotechnology, Biology and Medicine , 2016, 12(8): 2283-2290.

DOI：10.1016/j.nano.2016.06.013

• Nanotopography
• Nanogrooves
• Bone regeneration
• Rat femoral condyle
• Bone implants

In the present study, a method was developed to reproduce two nanogrooved patterns (groove width/ridge width/depth: 150/150/50 nm and 200/800/70 nm) into cylindrical epoxy resin implants, which were subsequently coated with 20 nm of titanium. Also, implants with a conventional surface roughness (R-q = 1.6 mu m) were produced. After cytocompatibility analysis of the produced surfaces, implants were installed into the femoral condyle of rats for 4 and 8 weeks. The histomorphometrical analysis of bone volume in a 100 mu m wide zone close to the implant surface showed that only for the 200/800 grooves the amount of bone increased significantly between 4 and 8 weeks of implantation. In addition, at the late time point only implants with the 200/800 pattern revealed a significantly higher bone volume compared to the rough controls. In conclusion, the 200/800 grooved pattern can positively influence bone volume adjacent to the implant surface, and should be evaluated and optimized in further (pre-) clinical studies.

• 出版日期2016-11