A multicolorimetric assay for rapid detection of Listeria monocytogenes based on the etching of gold nanorods

作者：Liu, Yushen; Wang, Juan; Zhao, Chao; Guo, Xiaoxiao; Song, Xiuling; Zhao, Wei; Liu, Sijie; Xu, Kun^*; Li, Juan

来源：Analytica Chimica Acta, 2019, 1048: 154-160.

DOI：10.1016/j.aca.2018.10.020

• Food safety
• Listeria monocytogenes
• Gold nanorods
• Multicolorimetric detection
• Immunomagnetic separation
• Artificial enzyme

Listeria monocytogenes (L. monocytogenes) is one of the most common food-borne pathogens. The authors describe a sensitive and reliable multicolorimetric assay for L. monocytogenes using a sensing system based on TMB2+ etching of gold nanorods. Apt-MNP was used as the capture probe, and IgY-BSA-MnO2 NPs was chosen as an oxidase-like nano-artificial enzyme to oxidize TMB to generate TMB2+. Under the optimized conditions, the longitudinal shift of localized surface plasmon resonances had a linear correlation with the L. monocytogenes concentration in the range between 10 to 10(6) cfu mL(-1). Meanwhile, the sensing system can generate vivid color responses as colorful as a rainbow, and the limit of detection is as low as 10 cfu mL(-1) at a glance. Recoveries ranging from 97.4 to 101.3% are found when analyzing spiked food samples without pre-enrichment. In our perception, it shows promise in rapid instrumental and on-site visual detection of L. monocytogenes.

• 出版日期2019-2-7
• 单位吉林大学