ANALYSIS OF ORGANOPHOSPHORUS PESTICIDES IN VEGETABLE SAMPLES BY HOLLOW FIBER LIQUID PHASE MICROEXTRACTION COUPLED WITH GAS CHROMATOGRAPHY-ELECTRON CAPTURE DETECTION

作者：Sanagi Mohd Marsin; Ghani Nurul Farhana Yanti A; Miskam Mazidatulakmam; Ibrahim Wan Aini Wan; Aboul Enein Hassan Y^*

来源：Journal of Liquid Chromatography & Related Technologies, 2010, 33(5): 693-703.

DOI：10.1080/10826071003608850

• GC-ECD
• hollow-fiber liquid phase microextraction
• organophosphorus pesticides

A method based on hollow fibre liquid phase microextraction (HF-LPME) coupled with gas chromatography electron capture detection (GC-ECD) has been developed for the determination of organophosphorus pesticides (OPPs) (chlorpyrifos and profenofos) in vegetable samples. In this method, a microsyringe needle with 1.5cm polypropylene hollow fibre containing a volume of organic acceptor phase (n-dodecane) was immersed in an aqueous donor solution, and at the completion of extraction, the acceptor phase was withdrawn and transferred to GC-ECD for analysis. The effects of extraction solvent, volume of acceptor phase, and volume of donor phase were investigated. The optimized conditions for HF-LPME of the selected OPPs were n-dodecane as organic solvent, 11mL of donor phase, and 3L of acceptor phase. The correlation coefficient (r2) of the calibration curves ranged from 0.998 to 0.999. The limits of detection (LOD) were between 0.099 and 0.128g/mL. The developed method provided excellent RSDs ranging from 0.54% to 8.00% and analyte recoveries ranging from 60.8% to 88.0%. This method was applied successfully for determination of organophosphorus pesticides in selected vegetables.

• 出版日期2010