A centrifugally actuated point-of-care testing system for the surface acoustic wave immunosensing of cardiac troponin I

作者：Lee Woochang; Jung Jaeyeon; Hahn Young Ki; Kim Sang Kyu; Lee Yeolho; Lee Joonhyung; Lee Tae Han; Park Jin Young; Seo Hyejung; Lee Jung Nam; Oh Jin Ho; Choi Youn Suk^*; Lee Soo Suk

来源：Analyst, 2013, 138(9): 2558-2566.

DOI：10.1039/c3an00182b

A fully automated point-of-care testing (POCT) system with a surface acoustic wave (SAW) immunosensor was developed for rapid and sensitive detection of cardiac troponin I (cTnI) in body fluid (plasma and whole blood). The assay, based on gold nanoparticle sandwich immunoassay and subsequent gold staining, was performed on the SAW immunosensor packaged inside a disposable microfluidic cartridge. The entire fluidic process, including plasma separation, reagent transport, metering, and mixing, was carried out by controlling the centrifugal force acting on the rotating cartridge and laser-irradiated ferrowax microvalves. On investigation of sensor response to various cTnI concentrations, the system exhibited a high performance with a detection limit of 6.7 pg mL(-1), and the coefficient of variation was less than 10% over the entire test range (10 pg mL(-1) to 25 ng mL(-1)). On comparing this POCT system with a clinically utilized system in a physical laboratory (Centaur(R) XP; Siemens), a correlation coefficient of 0.998 was found, validating the diagnostic capability of the SAW immunosensor.

• 出版日期2013