A facile method for studying interaction of rhodamine B and bovine serum albumin: Towards physical-binding mediated fluorescence labeling of proteins

作者：Ma Yu Xing; Zhong Rui Bo; Guo Jun; Liu Yu Shuang; Yuan Ming; Bai Zhi Jun; Liu Tao Tao; Zhao Xin Min; Zhang Feng^*

来源：Nuclear Science and Techniques, 2015, 26(6): 060502.

• Bovine serum albumin
• Rhodamine B
• Binding sites
• Scatchard equation
• Size exclusion chromatography

Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein (bovine serum albumin) and a typical fluorophore (rhodamine B) to demonstrate a direct labeling method just by physical adsorption. In combination with size exclusion chromatography and the Scartchard equation, we have developed a facile analysis method for calculating the binding constant and binding sites. The molecular docking method has been used to study the binding site in amino acid level.

• 出版日期2015-12
• 单位内蒙古农业大学