A DEVH-box RNA Helicase from Leishmania braziliensis is Associated to mRNA Cytoplasmic Granules

作者：Afonso Lehmann Raquel N; Thomasa Maria C; Santana Morales Maria A; Deniz Daniel; Lopez Manuel C; Valladares Basilio; Martinez Carretero Enrique^*

来源：Protist, 2015, 166(4): 457-467.

DOI：10.1016/j.protis.2015.07.002

• Trypanosomatids
• Leishmania
• RNA helicase
• mRNA granules

RNA helicases are ubiquitous enzymes that participate in almost all aspects of RNA processing, including RNA and RNA-protein complex remodelling. In trypanosomatids, which post-transcriptionally regulate gene expression, the formation of different kinds of ribonucleoprotein granules under stress conditions modulates the parasite's RNA metabolism. This paper describes the isolation of a putative DEVH-box RNA helicase produced by promastigotes of Leishmania braziliensis. Using a Cy3-labelled dT(30) oligo, FISH showed the localization of this protein to mRNA granules under starvation stress conditions. The central region of the protein was shown to be responsible for this behaviour.

• 出版日期2015-9