Action of an endo-beta-1,3(4)-glucanase on cellobiosyl unit structure in barley beta-1,3:1,4-glucan

作者：Kuge Takao; Nagoya Hiroki; Tryfona Theodora; Kurokawa Tsunemi; Yoshimi Yoshihisa; Dohmae Naoshi; Tsubaki Kazufumi; Dupree Paul; Tsumuraya Yoichi; Kotake Toshihisa^*

来源：Bioscience Biotechnology and Biochemistry, 2015, 79(11): 1810-1817.

DOI：10.1080/09168451.2015.1046365

• barley
• cellobiosyl unit
• endo-beta-1,3(4)-glucanase: beta-1,3:1,4-glucan
• MALDI-ToF/ToF-/MS/MS

beta-1,3:1,4-Glucan is a major cell wall component accumulating in endosperm and young tissues in grasses. The mixed linkage glucan is a linear polysaccharide mainly consisting of cellotriosyl and cellotetraosyl units linked through single beta-1,3glucosidic linkages, but it also contains minor structures such as cellobiosyl units. In this study, we examined the action of an endo-beta-1,3(4)-glucanase from Trichoderma sp. on a minor structure in barley beta-1,3:1,4-glucan. To find the minor structure on which the endo-beta-1,3(4)-glucanase acts, we prepared oligosaccharides from barley beta-1,3:1,4-glucan by endo-beta-1,4-glucanase digestion followed by purification by gel permeation and paper chromatography. The endo-beta-1,3(4)-glucanase appeared to hydrolyze an oligosaccharide with degree of polymerization 5, designated C5-b. Based on matrix-assisted laser desorption/ionization (MALDI) time-of-flight (ToF)/ToF-mass spectrometry (MS)/MS analysis, C5-b was identified as beta-Glc-1,3-beta-Glc-1,4-beta-Glc-1,3-beta-Glc-1,4-Glc including a cellobiosyl unit. The results indicate that a type of endo-beta-1,3(4)-glucanase acts on the cellobiosyl units of barley beta-1,3:1,4-glucan in an endo-manner.

• 出版日期2015
• 单位RIKEN