Actin, a reliable marker of internal control?

作者：Ruan Wenjing; Lai Maode^*

来源：Clinica Chimica Acta, 2007, 385(1-2): 1-5.

DOI：10.1016/j.cca.2007.07.003

• beta-actin
• varying expression

beta-actin is commonly used to normalize molecular expression studies due to its high conservation as an endogenous housekeeping gene. However, recent studies have shown that beta-actin expression can change in response to biochemical stimuli, during growth and differentiation, and in disease states. As can be expected, these phenomena substantially compromise the use of beta-actin as an internal reference marker. Under these conditions, varying expression of beta-actin likely indicates changed function for this maintenance molecule. Further studies exploring the function of actin in these environments will probably lead to a new integrative understanding of the roles of this housekeeping gene.

• 出版日期2007-10
• 单位浙江大学