Histone modification-mediated Lhx2 gene expression

作者：Park Key Sun; Kim Kee Kwang; Kim Kyoon Eon

来源：Biochemical and Biophysical Research Communications, 2012, 427(4): 718-724.

DOI：10.1016/j.bbrc.2012.09.123

• Lhx2
• E2F1
• HDAC3
• Histone deacetylation
• Trichostatin A

Lhx2, a member of LIM homeobox transcription factors, plays a key role in central nervous system (CNS) and embryonic tissue development. However, molecular mechanism of Lhx2 gene regulation remains largely unknown. Here, we identified and characterized a regulatory region of Lhx2 gene which mediates responses to two different signals such as inhibition of HDAC3 and stimulation by E2F1. In particular, the promoter region of -229 to -126 was responsible not only for basal expression but also for a inhibitor of histone deacetylase, trichostatin A (TSA)-mediated activation of Lhx2 gene. Intriguingly, transcription factor E2F1 also activates Lhx2 gene via direct binding to the same -229 to -126 region. Based on these observations, we could have demonstrated that E2F1 is necessary for TSA-mediated activation of Lhx2 gene and acetylation of histone 3 is involved in this event. This study provides evidence that the histone modification and E2F1 binding are integral parts of the mechanism for Lhx2 gene expression.

• 出版日期2012-11-2