A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au nanocomposite for horseradish peroxidase immobilization and biosensing

作者：Xu, JingJing; Peng, Ru; Ran, Qin; Xian, Yuezhong^*; Tian, Yuan; Jin, Litong

来源：Talanta, 2010, 82(4): 1511-1515.

DOI：10.1016/j.talanta.2010.07.033

• PEDOT-PSS conductive polymer
• Au nanopartiles
• Horseradish peroxidase
• Direct electrochemistry
• Hydrogen peroxide
• Biosensor

A highly soluble poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonic acid)/Au (PEDOT-PSS/Au) nanocomposite was prepared via one-step chemical synthesis and the matrix was characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and transmission electron microscope (TEM). Due to the excellent aqueous compatibility and biocompatibility, the PEDOT/PSS-Au nanocomposite can be used as biomaterial for enzymes immobilization. In this system, redox enzyme, horseradish peroxidase (HRP) was integrated with PEDOT/PSS-Au nanocomposite and the direct electron transfer of HRP was observed. Moreover, we find that the HRP/PEDOT-PSS/Au modified electrode shows excellent electrocatalytic ability for H2O2 and the formal Michaelis-Menten constant (K-m(app)) was 0.78 mmol/L The response currents have good linear relation with the concentrations of H2O2 with a linear range from 2.0 x 10(-7) to 3.8 x 10(-4) mol/L.

• 出版日期2010-9-15
• 单位华东师范大学