科研之友
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摘要
The selectivity of the resonance acoustic microbalance with naked-embedded quartz (RAMNE-Q) biosensor addition to its original features (label-free, high-sensitivity, and replacement-free) was improved by applying a sandwich assay. We succeeded in the real-time monitoring of a frequency change due to a series of reactions: the nonspecific adsorption of streptococcal protein G (SPG), the oriented immobilization of an anti-C-reactive protein (anti-CRP) antibody, the specific binding of CRP as the antigen, and the specific binding of another anti-CRP antibody as the detection antibody. The detection of the low concentrations of CRP (100 pg/ml) and the anti-CRP antibody (1 mu g/ml) was carried out repeatedly, and its repeatability was confirmed.
- 出版日期2013-7
