Dynamics of arachidonic acid mobilization by inflammatory cells

作者：Astudillo Alma M; Balgoma David; Balboa Maria A; Balsinde Jesus^*

来源：Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids, 2012, 1821(2): 249-256.

DOI：10.1016/j.bbalip.2011.11.006

• Arachidonic acid
• Phospholipid remodeling
• Eicosanoid metabolism
• Lipidomics
• Mass spectrometry

The development of mass spectrometry-based techniques is opening new insights into the understanding of arachidonic acid (AA) metabolism. AA incorporation, remodeling and release are collectively controlled by acyltransferases, phospholipases and transacylases that exquisitely regulate the distribution of AA between the different glycerophospholipid species and its mobilization during cellular stimulation. Traditionally, studies involving phospholipid AA metabolism were conducted by using radioactive precursors and scintillation counting from thin layer chromatography separations that provided only information about lipid classes. Today, the input of lipidomic approaches offers the possibility of characterizing and quantifying specific molecular species with great accuracy and within a biological context associated to protein and/or gene expression in a temporal frame. This review summarizes recent results applying mass spectrometry-based lipidomic approaches to the identification of AA-containing glycerophospholipids, phospholipid AA remodeling and synthesis of oxygenated metabolites.

• 出版日期2012-2