Apoptotic cell death in erythrocytes of p53-deficient medaka (Oryzias latipes) after gamma-irradiation

作者：Sayed Alaa El Din Hamid^*; Watanabe Asaka Tomomi; Oda Shoji; Mitani Hiroshi

来源：International Journal of Radiation Biology, 2016, 92(10): 572-576.

DOI：10.1080/09553002.2016.1222091

• Apoptosis
• erythrocytes
• 4 Gy
• medaka
• p53

Purpose: Previous studies have examined the effects of gamma-irradiation (gamma-IR) on wild-type and p53 mutant Medaka (Oryzias latipes) 24 hours after irradiation and in the present work, apoptosis and alterations in erythrocytes of 4, 8 and 24 h and 14 days after gamma-ray irradiation were reported as geno-toxic biomarkers of gamma-irradiation.
Materials and methods: Sexually mature wild-type, WT (Hd-rR) and p53(-/-) adult female medaka (O. latipes) were exposed to 4 Gy dose of gamma-IR and sampling were collected after 4, 8 and 24h and 14 days.
Results: Apoptosis and morphological alterations were observed from 4h after irradiation and remarkably increased 8 h after irradiation in the wild-type. Apoptotic cell death has been observed 8 h after irradiation most prominently but subtle in p53 mutant medaka. All these phenotypes were recovered 14 days after irradiation in both strains. Although no micronuclei were seen in any group, nuclear abnormalities were observed in red blood cells. Both apoptosis and morphological alterations in erythrocytes were decreased after 24 and 14 days after gamma-irradiation.
Conclusions: We conclude that apoptosis and malformations caused by 4 Gy gamma-irradiation in the erythrocytes of medaka fish occurs from 4-24h and the initial response until 8h was p53-dependent.

• 出版日期2016-10