科研之友
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摘要
Calcium release units (CRUs) and mitochondria control myoplasmic [Ca2+] levels and ATP production in muscle, respectively. We recently reported that these two organelles are structurally connected by tethers, which promote proximity and proper Ca2+ signaling. Here we show that disposition, ultrastructure, and density of CRUs and mitochondria and their reciprocal association are compromised in muscle from aged mice. Specifically, the density of CRUs and mitochondria is decreased in muscle fibers from aged (>24 months) vs. adult (3-12 months), with an increased percentage of mitochondria being damaged and misplaced from their normal triadic position. A significant reduction in tether (13.8+/-0.4 vs. 5.5+/-0.3 tethers/100 mu m(2)) and CRU-mitochondrial pair density (37.4+/-0.8 vs. 27.0+/-0.7 pairs/100 mu m(2)) was also observed in aged mice. In addition, myoplasmic Ca2+ transient (1.68+/-0.08 vs 1.37+/-0.03) and mitochondrial Ca2+ uptake (9.6+/-0.050 vs 6.58+/-0.54) during repetitive high frequency tetanic stimulation were significantly decreased. Finally oxidative stress, assessed from levels of 3-nitrotyrosine (3-NT), Cu/Zn superoxide-dismutase (SOD1) and Mn superoxide dismutase (SOD2) expression, were significantly increased in aged mice. The reduced association between CRUs and mitochondria with aging may contribute to impaired cross-talk between the two organelles, possibly resulting in reduced efficiency in activity-dependent ATP production and, thus, to age-dependent decline of skeletal muscle performance.
- 出版日期2015-11-3
