Ultrasonication on a microfluidic chip to lyse single and multiple Pseudo-nitzschia for marine biotoxin analysis

作者：Wu, Chunsheng; Lillehoj, Peter B.; Sabet, Leyla; Wang, Ping; Ho, Chih-Ming^*

来源：Biotechnology Journal, 2011, 6(2): 150-155.

DOI：10.1002/biot.201000224

• Hydrodynamic cell trapping
• Microfluidics
• Pseudo-nitzschia
• Ultrasonic cell lysis

We present a microfluidic platform, which provides a simple and efficient means for handling and processing Pseudo-nitzschia, a neurotoxin-producing marine algae. Currently, analyzing the production of such toxins is complicated by multiple environmental factors and high variability among individual Pseudo-nitzschia species. To address this issue, we developed a device that can precisely trap single and multiple cells for subsequent lysis to extract relevant intracellular molecules. Our results show a cell trapping efficiency of up to 96%, which is achieved by hydrodynamic flow focusing. Additionally, complete cell lysis via ultrasonication can be accomplished within a few seconds. This platform can be applied to other algae and non-algae cell types with minimal modification, thus providing a valuable tool for studying biological intracellular mechanisms at the single and multi-cell level.

• 出版日期2011-2
• 单位浙江大学