Development of a synthetic pathway to convert glucose to hydrogen using cell free extracts

作者：Lu Franklin; Smith Phillip R; Mehta Kunal; Swartz James R

来源：International Journal of Hydrogen Energy, 2015, 40(30): 9113-9124.

DOI：10.1016/j.ijhydene.2015.05.121

• Biohydrogen
• Hydrogenase
• Synthetic enzyme pathway
• Ferredoxin-NADP(+)-reductase
• Ferredoxin
• Cell-free metabolic engineering

Sustainable production of biochemicals from cellulosic biomass is an attractive alternative to chemical production from fossil fuels. We describe the further development of a three protein synthetic pathway to convert NADPH and H+ to hydrogen using a ferredoxin-NADP(+) reductase, a ferredoxin, and the [FeFe] hydrogenase from Clostridium pasteurianurn at a rate greater than 14 mmol H-2 L-1 hr(-1) using natural enzymes. We also demonstrate the feasibility of coupling this pathway to a cell-free extract to convert glucose to hydrogen in a potentially cost effective manner. Both of these accomplishments serve as the basis for further engineering to optimize both the yield and productivity of a low-cost cell-free process for the production of highly reduced biochemicals.

• 出版日期2015-8-10