Histological features of human papilloma virus 16 and its association with the development and progression of anal squamous cell carcinoma

作者：Kagawa Ryuzaburo; Yamaguchi Tetsuya; Furuta Reiko

来源：Surgery Today, 2006, 36(10): 885-891.

DOI：10.1007/s00595-006-3266-x

• HPV16
• anal squamous cell carcinoma
• polymerase chain reaction
• in situ hybridization
• Duchenne muscular dystrophy

Purpose. To investigate the development of anal squamous cell carcinoma (SCC) and the expression patterns of human papillomavirus (HPV).
Methods. We performed typing of HPV-DNA using a polymerase chain reaction (PCR), and amplified the Duchenne muscular dystrophy (DMD) genes simultaneously, to investigate the tumor DNA state. The expression patterns of HPV in the cancer cell nuclei was investigated by in situ hybridization (ISH) using HPV probes.
Results. Amplification of DMD genes was confirmed in 8 of 20 patients with anal SCC, suggesting that tumor DNA was preserved in these patients. In seven of these eight patients, only HPV16 was detected by both PCR and ISH, suggesting HPV16-induced carcinogenesis. In two patients with carcinoma in situ (CIS), the cancer cells showed only a diffuse pattern (DP), and in two patients with invasive cancer, the cancer cell showed only an oligo-dot pattern (OP). In one patient with lesions ranging from CIS to invasive cancer, the histologic features varied in each area, from DP to OP. This change originated in the deep part of the microinvasive area.
Conclusions. These findings show that HPV16 infection is closely involved in the development of anal SCC and suggest that the change in the genome occurs at the stage of microinvasive cancer.

• 出版日期2006-10