NUCLEOTIDE-SEQUENCE OF AN ENDO-BETA-1,4-GLUCANASE GENE FROM BACILLUS-SUBTILIS CK-2

作者：LINDAHL V^*; AA K; TRONSMO A

来源：Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 1994, 66(4): 327-332.

DOI：10.1007/BF00882768

• ENDO-BETA-1,4-GLUCANASE GENE
• BACILLUS SUBTILIS

The gene encoding endo-beta-1,4-glucanase in Bacillus subtilis CK-2 was cloned into Escherichia coli DH5 alpha, and the nucleotide sequence determined. The 1500 bp gene encodes a protein of 499 amino-acid residues with a calculated molecular mass of 55 261, and is equipped with a typical B. subsilis signal peptide. Nucleotide sequence comparison revealed only 2 basepairs deviation between this gene and the endo-beta-1,4-glucanase gene of B. subtilis PAP115, and 93% to 95% homology was found between the amino acid sequences of these enzymes and other B. subtilis endo-beta-1,4-glucanases. Regions of similarity were also observed between the carboxy-terminal part of these enzymes and the part of the B. lautus PL236 celA enzyme constituting the cellulose-binding domain.

• 出版日期1994