Anti-inflammatory and immunoregulatory effects of flax-seed oil and Lactobacillus plantarum - Biocenol (TM) LP96 in gnotobiotic pigs challenged with enterotoxigenic Escherichia coli

作者：Chytilova M; Mudronova D^*; Nemcova R; Gancarcikova S; Buleca V; Koscova J; Tracikova L

来源：Research in Veterinary Science, 2013, 95(1): 103-109.

DOI：10.1016/j.rvsc.2013.02.002

• Lactobacilli
• Flax-seed oil
• ETEC
• Gnotobiotic pig
• qRT-PCR
• Gene expression

The aim of this study was to determine the immune response after preventive administration of flax-seed oil (rich in n-3 PUFAs) or probiotic strain Lactobacillus plantarum - Biocenol (TM) LP96 or their combination in the jejunum of ETEC-challenged gnotobiotic pigs. Subsequently, gene expression of selected cytokines, phagocytic activity of leukocyes from peripheral blood and percentage of CD2(+), CD4(+), CD8(+) and CD4(+-)CD25(+) lymphocytes in jejunal mucosa were evaluated. Our results showed that combined treatment down-regulates IL-1 alpha and IL-8 gene expression, up-regulates IFN-gamma and tends to regulate inflammation induced by ETEC through cytokine IL-10. In general, changes in cytokine gene expression correlated with the proportions of immune cells isolated from the same part of the jejunal mucosa. Results indicate that probiotic L plantarum in combination with flax-seed oil rich in n-3 PUFAs has anti-inflammatory properties, stimulates Th1-mediated cell immunity and phagocytosis, and tends to regulate the inflammatory response induced by ETEC.

• 出版日期2013-8