An investigation on the subcellular distribution and compartmentalization of uranium in Phaseolus vulgaris L.

作者：Nie Xiaoqin; Dong Faqin; Liu Ning^*; Liu Mingxue; Zhang Wei; Sun Shiyong; Yang Jie

来源：Journal of Radioanalytical and Nuclear Chemistry, 2014, 299(3): 1351-1357.

DOI：10.1007/s10967-013-2859-2

• Uranium
• Subcellular distribution
• Phaseolus vulgaris L.

Phaseolus vulgaris L. (bean) is a promising species for uranium rhizofiltration with high tolerance and accumulation ability. To further understand the mechanisms involved in uranium tolerance and detoxification, the present study investigated subcellular distribution and compartmentalization of uranium in bean. Subcellular fractionation of uranium containing tissues indicated that both in roots and shoots, the concentration of uranium in each subcellular fractions increased evidently with increasing solution uranium level, and the majority of uranium was located in cytosol and cell wall fraction, while a minor part of uranium associated with the organelle fraction. Meanwhile, with uranium concentration increasing from 100 to 1,000 mu M, the proportion of uranium distribution in cytosol fraction was decreased but it was increased in cell wall fraction. However, the proportion of uranium distribution in organelle fraction is always less than 5 %. These results suggest that stored in the cytosol (such as uranium compartmentalization with organoligands in vacuole) and bound to the cell walls (may be integrated with polyose and protein) might play an important role in tolerance and detoxification of uranium in bean.

• 出版日期2014-3
• 单位四川大学