ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing

作者：Chen Xingqi; Shen Ying; Draper Will; Buenrostro Jason D; Litzenburger Ulrike; Cho Seung Woo; Satpathy Ansuman T; Carter Ava C; Ghosh Rajarshi P; East Seletsky Alexandra; Doudna Jennifer A; Greenleaf William J; Liphardt Jan T; Changsites Howard Y^*

来源：Nature Methods, 2016, 13(12): 1013-+.

DOI：10.1038/NMETH.4031

Spatial organization of the genome plays a central role in gene expression, DNA replication, and repair. But current epigenomic approaches largely map DNA regulatory elements outside of the native context of the nucleus. Here we report assay of transposase-accessible chromatin with visualization (ATAC-see), a transposase-mediated imaging technology that employs direct imaging of the accessible genome in situ, cell sorting, and deep sequencing to reveal the identity of the imaged elements. ATAC-see revealed the cell-type-specific spatial organization of the accessible genome and the coordinated process of neutrophil chromatin extrusion, termed NETosis. Integration of ATAC-see with flow cytometry enables automated quantitation and prospective cell isolation as a function of chromatin accessibility, and it reveals a cell-cycle dependence of chromatin accessibility that is especially dynamic in G1 phase. The integration of imaging and epigenomics provides a general and scalable approach for deciphering the spatiotemporal architecture of gene control.

• 出版日期2016-12