Analysis of genetic variability in Aquilaria malaccensis from Bramhaputra valley, Assam, India using ISSR markers

作者：Banu Sofia^*; Baruah Darshana; Bhagwat Rasika M; Sarkar Purabi; Bhowmick Ananya; Kadoo Narendra Y

来源：Flora, 2015, 217: 24-32.

DOI：10.1016/j.flora.2015.09.007

• Agarwood
• Endemic
• Genetic diversity
• ISSR
• UPGMA
• Conservation

Aquilaria malaccensis is a threatened agarwood producing plant with high ecological and economic importance. In the present study, we used inter-simple sequence repeat (ISSR) markers to analyze the pattern of genetic variation and differentiation among seven populations comprising 53 individuals of the species, dispersed across the biodiversity rich Brahmaputra valley of Assam, India. Of the 50 ISSR primers initially evaluated, 16 primers generated distinct and reproducible DNA amplicons. Using these 16 primers, 166 discernible DNA amplicons were generated of which 155(93.4%) were polymorphic. Total genotype diversity among the populations (H-T) was 0.25 (0.03), while the within population diversity (H-s) was 0.14 (0.02). The mean coefficient of gene differentiation (G(ST)) was as high as 0.44, resulting in an estimate for gene flow (N-m) of 0.63, indicating moderate genetic differentiation among the populations of A. malaccensis. Analysis of molecular variance (AMOVA) indicated that 38% variance was attributable to among-populations diversity and the rest (62%) to differences within populations. The pattern of genetic diversity observed in A. malaccensis indicated existence of a diverse array of genotypes, implicating the need for preservation of every population to maintain genetic variability in this highly important and vulnerable tree species.

• 出版日期2015