A New Method for Producing Biologically Active Nanocomplexes by a Noncovalent Conjugation of Proteins with Viral Particles

作者：Yaroslavov A A; Kaplan I B^*; Erokhina T N; Morozov S Yu; Solovyev A G; Leshchiner A D; Rakhnyanskaya A A; Malinin A S; Stepanova L A; Kiselev O I; Atabekov J G

来源：Russian Journal of Bioorganic Chemistry, 2011, 37(4): 441-447.

DOI：10.1134/S1068162011040169

• nanocomplexes
• viral particles
• conjugation with proteins
• polycation
• hemagglutinin
• immunization

A new method for noncovalent immobilization of a peptide epitope on the virion surface was developed to simplify and standardize the procedures for producing viral nanocomplexes. The efficacy of this approach was demonstrated by the example of a model system comprising the tobacco mosaic virus, synthetic cationic polymer poly-N-ethyl-4-vinylpyridinium bromide, and a model polypeptide. The principle of sequential adsorption, underlying production of the triple system virion-polycation-protein, was used for electrostatic immobilization of a recombinant hydrophilic fragment of the influenza virus hemagglutinin (Flu1-3) on the virion surface. The method provided for a significant increase in the immunogenic activity of this potential artificial vaccine protein.

• 出版日期2011-7