Analytic lymph node number establishes staging accuracy by occult tumor burden in colorectal cancer

作者：Hyslop Terry; Weinberg David S; Schulz Stephanie; Barkun Alan; Waldman Scott A^*

来源：Journal of Surgical Oncology, 2012, 106(1): 24-30.

DOI：10.1002/jso.23051

• lymph node collection
• colorectal cancer
• molecular staging
• occult tumor burden
• guanylyl cyclase C

Background and Objectives Recurrence in lymph node-negative (pN0) colorectal cancer suggests the presence of undetected occult metastases. Occult tumor burden in nodes estimated by GUCY2C RT-qPCR predicts risk of disease recurrence. This study explored the impact of the number of nodes analyzed by RT-qPCR (analytic) on the prognostic utility of occult tumor burden. Methods Lymph nodes (range: 2-159) from 282 prospectively enrolled pN0 colorectal cancer patients, followed for a median of 24 months (range: 263), were analyzed by GUCY2C RT-qPCR. Prognostic risk categorization defined using occult tumor burden was the primary outcome measure. Association of prognostic variables and risk category were defined by multivariable polytomous and semi-parametric polytomous logistic regression. Results Occult tumor burden stratified this pN0 cohort into categories of low (60%; recurrence rate (RR)?=?2.3% [95% CI 0.14.5%]), intermediate (31%; RR?=?33.3% [23.744.1%]), and high (9%; RR?=?68.0% [46.585.1%], P?%26lt;?0.001) risk of recurrence. Beyond race and T stage, the number of analytic nodes was an independent marker of risk category (P?%26lt;?0.001). When %26gt;12 nodes were analyzed, occult tumor burden almost completely resolved prognostic risk classification of pN0 patients. Conclusions The prognostic utility of occult tumor burden assessed by GUCY2C RT-qPCR is dependent on the number of analytic lymph nodes. J. Surg. Oncol. 2012; 106:2430.

• 出版日期2012-7